ulk1 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc ulk1
Ulk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ulk1/ULK1+Rabbit+mAb/10__1002_slash_fsn3__72313-91-37-6
Average 96 stars, based on 1157 article reviews

ulk1 - by Bioz Stars, 2026-11

96/100 stars

Images

Bioprocessing:

Article Title: SLC29A1 /ENT1 and SLC29A3 /ENT3 differentially regulate autophagy.
Article Snippet: Rabbit polyclonal antihuman SLC28A1/ENT1 antibody (ab135756) was purchased from Abcam. .. Rabbit monoclonal antibodies against MAP1LC3B/LC3B (3868S), phospho-MTOR (5536S), total MTOR (2983S), phospho-RPS6KB/S6K (9234S), total RPS6KB/S6K (2708S), phospho-EIF4EBP1/4E-BP1 (2855S), total EIF4EBP1/4E-BP1 (9644S), phospho-ULK1 (Ser555, 5869S; Ser757, 14202S), total ULK1 (6439S), phosphoACACA/ACC (11818S), and rabbit polyclonal antibodies against phospho-PRKAA/AMPK (2531S), total PRKAA/ AMPK (2532S), and total ACACA/ACC (3662S) were obtained from Cell Signaling Technology. .. Mouse monoclonal anti-SQSTM1/p62 antibody (610833) was from BD Biosciences.

Cell-Signaling:

Article Title: SGLT2 inhibition induces autophagic flux blockade and sensitizes pancreatic cancer to EGFR-targeted therapy
Article Snippet: After incubation with HRP-conjugated secondary antibodies (Abclonal) for 1 h at room temperature, signals were detected using the enhanced chemiluminescence (ECL) reagent. .. The following primary antibodies were used: SGLT2 (Invitrogen, PA5-101893, 1:1000; #14120, CST, 1:1000), Phospho-AMPKα (Thr172; #2535, Cell Signaling Technology, CST, Danvers, MA, USA; 1:1000), AMPKα1/α2 ( A27099 , Abclonal, Wuhan, China; 1:2000), Phospho-ULK1 (Ser555, #5869, CST; 1:1000), ULK1 (#8054, CST; 1:1000), SQSTM1/p62 (#5114, CST; 1:1000), LC3B (#3868, CST; 1:1000), Bax (#2772, CST; 1:1000), Bcl-2 (#3498, CST; 1:1000), Cleaved Caspase-3 (#9661, CST; 1:1000), Caspase-3 (#14220, CST; 1:1000), EGFR (A11351, Abclonal; 1:2000), and Phospho-EGFR (Tyr1068, AP0994, Abclonal; 1:1000; #3777, CST, 1:1000). .. β-Actin (AC026, Abclonal; 1:80000) was used as the loading control.

Article Title: A Plant‐Derived Functional Oral Formulation Improves Growth Performance and Hepatic Metabolic Function in Poultry Under Heat Stress: A Nutritional Intervention Study
Article Snippet: .. After blocking with 5% skim milk (CST, USA) for 2 h, membranes were incubated with primary antibodies against HSP70 (8242), Atg4B (13507), Atg5 (12994), Atg7 (8558), Atg12 (4180), Atg16L1 (8089), LC3I/II (12741), mTOR (2983), p- mTOR (5536), ULK1 (8054), p- ULK1 (14202), Beclin1 (3495), and GAPDH (5174) (all from Cell Signaling Technology, Danvers, MA, USA; all at 1:1000 dilution). .. After secondary antibodies (Proteintech, USA) were incubated, the membranes were visualized with ECL kits (Bio- Rad, USA) and the chemiluminescence image system (Tanon, Shanghai, China), and the gray level of the protein expression was quantified by Image J software.

Immunofluorescence:

Article Title: Damage-sensing recruitment of a lipid phosphatase couples lysosomal membrane repair to proteostatic adaptation
Article Snippet: .. GAPDH (glyceraldehyde-3-phosphate dehydrogenase; mouse, Sigma-Aldrich, G8795, 1:4000); actin ( mouse, Sigma-Aldrich, Cat# A5441, 1:4000);Phospho-p70 S6 Kinase (Thr389) (rabbit, Cell signaling, Cat# 9205L,1:500), p70 S6 Kinase Antibody (rabbit, Cell Signaling, 9202L, 1:1000); PI4K2A (homemade); MTMR14 (homemade); dsRed/mScarlet (rabbit, Clontech, Cat# 632496, 1:1000); pULK(S757) (rabbit, Cell signaling, Cat# 6888, 1:1000); ULK1(rabbit, Cell signaling, Cat# 8054, 1:1000); p4E-BP1(S65) (rabbit, Cell signaling, Cat# 13443; 1:500); 4EBP1 (rabbit, Cell signaling, Cat# 9644; 1:500); pTFEB(S211) (rabbit, Cell signaling, Cat# 37681, 1:1000); TFEB (rabbit, Biomol, Cat# A303-673A, 1:1000); Anti-mouse HRP (Jackson, Cat# 115-035-003, 1:4000); Anti-rabbit HRP (Jackson, Cat# 111-035-003, 1:4000); Anti-mouse Alexa568 (IgM-specific) (Thermo, Cat# A21043, immunofluorescence 1:400); PtdIns(4)P (Echelon, Cat# z-p004, immunofluorescence 1:200); a-RXRXX[S/T] (Cell Signaling, Cat# 10001, 1:500); pS6 (Cell Signaling, Cat# 2215, 1:1000); pS6(S240/244) (Cell Signaling, Cat# 5364, 1:1000); puromycin (Kerafast, Cat# Kf-Ab02366-23.0, WB 1:500, immunofluorescence 1:2000); CHMP4B (Proteintech, 13683-1-AP, immunofluorescence 1:200); IST1 (proteintech, Cat# 19842-1-AP, immunofluorescence 1:200); Alix (Biolegend, Cat# 634501); PSMB5 (Thermo, Cat# PA1-977, immunofluorescence 1:200); Anti-mouse CF568 (Biotium, Cat# 20109-0.25ml, immunofluorescence 1:1000); Anti-rabbit Alexa 647 (Thermo, Cat# A31573, immunofluorescence 1:1000); Anti-rabbit Alexa 568 (Thermo/ Molecular Probes, Cat# A11036, immunofluorescence 1:1000); Anti-mouse Alexa 647 (life, Cat# A21238, immunofluorescence 1:1000); anti-Mouse IRDye 680 CW (LICOR, P/N 925-68070, 1:4000); anti-Rabbit IRDye 680 CW (LICOR, P/N 925-68180, 1:4000). .. YFP-MTMR14WT and YFP-MTMR14(C330S) plasimds as a gift from Jocelyn Laporte (IGBMC Strasbourg, France), eGFP-EqtSM , eGFP-2xFYVE (in-house), pmCherry-Gal3 (addgene #85662), eGFP-OSBP-PH (in-house), GFP-SNXA was a gift from Jason S. King (University of Sheffield, UK), eGFP-KIF16Btail (addgene #134624), mCherry-MTMR14 (in-house), pmCherry-P4M-SidMx2 (in-house), pRK5-eGFP-Tau P301L (addgene # 46908), eGFP-MTM1 (in-house), eGFP-MTMR1 (in-house), eGFP-MTMR2 (in-house), eGFP-MTMR6 (in-house), EGFP-MTMR7 (in-house), eGFP-MTMR8 (in-house), pMRX-IB-TMEM192-mKeima (addgene #215359), HA-PI4K2A (in-house), eGFP-Ubiquitin (addgene #187910), mCherry-MTMR14 ΔN (delete 1-68) (in-house), mCherry-MTMR14 ΔC (delete 406-650) (in-house), mCh-MTMR14 Δ620-650 (in-house), mCh-MTMR14 Δ586-650 (in-house), CFP-MTMR14 C-terminus (delete 1-570) (in-house), mCh-MTMR14 C-terminus (delete 1-570) (in-house), mCh-MTMR14 C-terminus 7xmut (L590E L591E A592S A593S L594E F608E Y612E) (in-house), mCh-MTMR14 FL 7xmut (L590E L591E A592S A593S L594E F608E Y612E) (in-house).

Western Blot:

Blocking Assay:

Incubation:

other:

Article Title: Constitutive AMPK activation prevents hepatocellular carcinoma development through inhibition of HNF4α activity
Article Snippet: ULK1 , Cell Signaling Technology , 8054.

Article Title: Design, synthesis and biological evaluation of benzimidazole carbamide derivatives - potential autophagy inducers.
Article Snippet: Herein, we report a convenient synthetic approach and an in vitro evaluation of benzimidazole carbamide derivatives as potential autophagy activators.. Two compounds exhibited autophagy-inducing activity at concentrations ≥100 μM.. The most favorable activity–toxicity profile was observed for a benzimidazole derivative lacking a carbamide fragment.

Review